Antioxidant Activity Test Of Ethanol And N-Hexane Extracts Of Red Teak Leaves (Tectona Grandis L. F) Using The Dpph Method

Abstract

The red teak plant (Tectona grandis L.f) is known as a medicinal plant with potential as a natural source of antioxidants. The active compounds found in red teak leaves, such as flavonoids and tannins, contribute to their antioxidant activity. This study aims to determine the antioxidant activity and IC₅₀ values of ethanol and n-hexane extracts of red teak leaves. The research was conducted experimentally through extraction using 96% ethanol and n-hexane solvents, followed by antioxidant activity testing using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, analyzed with a UV-Vis spectrophotometer. Phytochemical tests showed that the 96% ethanol extract contained flavonoids, saponins, tannins, alkaloids, and steroids, while the n-hexane extract contained flavonoids, tannins, alkaloids, and steroids. Antioxidant activity analysis revealed that the 96% ethanol extract had an IC₅₀ value of 29.896 ppm, categorized as very strong, while the n-hexane extract had an IC₅₀ value of 116.169 ppm, categorized as moderate. As a comparison, vitamin C exhibited very strong antioxidant activity with an IC₅₀ value of 5.717 ppm.